
Articles in refereed journals
Swanston, T., T. Varney, I.
Coulthard, R. Feng, B. Bewer, R. Murphy, C. Hennig, and D. Cooper. Element
localization in archaeological bone using synchrotron radiation x-ray
fluorescence: Identification of biogenic uptake. Journal of Archaeological
Science. (Accepted, Jan. 26th; JASC11-608.)
Swanston, T., Y. Carter, C. Hopkins, E.G. Walker, and D. Cooper. Developmental Fusion of the Malleus and Incus in a Late 19th-Century Case of Aural Atresia. International Journal of Osteoarchaeology. Article first published online : 15 SEP 2011, DOI: 10.1002/oa.1286
2011. The Characterization of Helicobacter pylori DNA Associated with Ancient Human Remains Recovered from a Canadian Glacier. PLoS ONE 6(2): e16864. doi:10.1371/journal.pone.0016864Monsalve MV., E. Humphrey E, DC. Walker, C. Cheng, T. Swanston, H. Deneer, P. Hazelton, LG. Oda, E. Kahila, G. Bar-Gal and M. Spigelman. (2008) Report of a Multidisciplinary Workup of the Kwäday Dän Ts’ìnchi Human Remains from Canada: Micro-organisms analyses. In: P. Atoche, C. Rodríguez and M.A. Ramírez (eds.) Mummies and Science. World Mummies Research. Proceedings of the VI Congress on Mummy Studies (Teguise, Lanzarote, 2007), pp. 407-414. Santa Cruz de Tenerife.
In paleopathology,
the assessment of disease occurs through macroscopic observation, which is dependent
on the preservation of the sample and the experience of the observer. Many disease events do not leave any visible
signatures and therefore go undetected.
The relatively new field of paleomicrobiology incorporates molecular
techniques where microbial DNA, if present, is amplified from an archaeological
sample. The identification of genetic
material from micro-organisms, including bacteria and viruses, can confirm a
diagnosis that was originally based on visible osteological or mummified tissue
changes. Even more promising is the
capability of molecular technology to detect microbial DNA evidence of disease
processes that were not visibly evident.
In 1999, human remains were
discovered eroding out of a glacier in northern British
Columbia, Canada
on the traditional territory of the Champagne
and Aishihik First Nations. The
Aboriginal elders named the site Kwäday Dän Ts’ìnchi, which means ‘long ago
person found.’ Radiocarbon testing of
bone collagen and artifacts from the site suggested a time-frame of
approximately AD 1670 to 1850, which is either pre-European contact or early
post-contact for that area. The
tissues of the ancient individual were analyzed specifically for genetic evidence of M. tuberculosis and H. pylori to identify partial health status and determine if a
connection could be made to strains associated with European populations to
clarify whether the site was pre or post-European contact.
Through polymerase chain reaction (PCR) testing of the individual’s tissues with primers specific for the IS6100 insertion sequence, TbD1, and Rv3479, katG and gyrB genes, evidence of a possible latent tuberculosis infection was identified. Genetic characterization of the katG gene associated with the ancient M. tuberculosis strain revealed a potential connection with European strains. Amplification and sequencing of the gyrB gene fragment indicated the presence of two alleles that may have been the result of a selective pressure.
PCR testing of the individual’s stomach tissue with specific primers for regions with the vacA gene resulted in a positive identification of H. pylori DNA. Genetic characterization of this virulence-associated gene indicated that the strain contained a vacA signal (s) region s2 allele. This allele is more commonly identified in Western strains that do not cause disease, which suggests that the individual had no gastric symptoms and that European strains were present in northwestern Canada at that time. The vacA middle (m) region contained a hybrid m2a/m1d sequence. Modern hybrids are rare but they have been identified in Asian strains. Studies have shown that the m2a allele is more common in Western strains. A phylogenetic analysis identified that the m1d region clusters with previously published novel strains associated with Aboriginal individuals that are closely related to Asian strains. This indicates a past connection between the ancient individual and his ancestors who arrived in the New World from Asia thousands of years ago.

In the fall of 1999, human skeletal remains and historic artifacts were discovered on private farmland approximately two kilometres south of the Town of Battleford, Saskatchewan. Document searches and a ground-penetrating radar survey of the property resulted in the discovery that the land was once used as a cemetery for the Catholic Church of St. Vital during the years of 1879 to 1885. Numerous interest groups were brought together in the process of handling this sensitive situation, including the landowners, the Heritage Resource Branch of the Department of Saskatchewan Culture, Youth and Recreation, the Rural Municipality of Battle River, the Roman Catholic Diocese of Prince Albert, and the Battleford Tribal Council. A decision was made to relocate the burials to the current town cemetery. In the meantime, permission was granted for the University of Saskatchewan to play the lead role in the excavation and analysis process. The partial and complete skeletal remains of thirty individuals were recovered, and in addition to a basic osteology analysis of the individuals that included sex determination, age at death and population affinity, a detailed look at the pathological conditions was also undertaken. The document and artifact analyses was the subject of a separate thesis by Colette Hopkins.
To teach is to have the privilege and responsibility of passing on knowledge to others. Effective communication in the classroom is necessary for the development of a comfortable and respectful atmosphere that is conducive to discussions and learning. Students should feel free to ask questions in a setting where they are not judged. They should also have the expectation that I will facilitate their learning process in a fair and straightforward manner with clearly stated course objectives, and they have the right to expect that I will be up-to-date on the subject matter and teach with enthusiasm! I have the expectation that my students will attend class regularly and complete the requirements of the course. Through mutual effort, we are capable of growing and learning from each other.